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. 2022 May 20;298(6):102057. doi: 10.1016/j.jbc.2022.102057

Figure 1.

Figure 1

Role of the C-terminal tail of GHSR1a β-arrestin recruitment.A, schematic representation of the ICL3 and C-terminal region of GHSR1a highlighting the phosphorylation sites mutated to alanine in the GHSR1aΔpA mutant. B, ghrelin stimulated β-arrestin2 recruitment to GHSR1a and GHSR1aΔpA with and without MRAP2. Arrow indicates ghrelin injection time. C, ghrelin stimulated β-arrestin1 recruitment to GHSR1a and GHSR1aΔpA with and without MRAP2. D, ghrelin-stimulated IP1 accumulation assay in cells transfected with GHSR1a with and without MRAP2. E, ghrelin-stimulated IP1 accumulation assay in cells transfected with GHSR1aΔpA with and without MRAP2. F, detection of GHSR1a and GHSR1aΔpA with and without MRAP2 by ELISA in nonpermeabilized cells. G, detection of GHSR1a and GHSR1aΔpA with and without MRAP2 by ELISA in permeabilized cells. H, schematic representation of the ICL3 and C-terminal region of GHSR1aΔc.t. I, ghrelin stimulated β-arrestin2 recruitment to GHSR1a and GHSR1aΔc.t with and without MRAP2. J, ghrelin stimulated β-arrestin1 recruitment to GHSR1a and GHSR1aΔc.t with and without MRAP2. K, detection of GHSR1a and GHSR1aΔc.t with and without MRAP2 by ELISA in nonpermeabilized cells. L, detection of GHSR1a and GHSR1aΔc.t with and without MRAP2 by ELISA in permeabilized cells. Data are the mean ± SEM of three independent experiments performed in triplicate. ∗∗∗p < 0.001. GHSR1a, growth hormone secretagogue receptor 1a; ICL3, third intracellular loop.