Skip to main content
. 2022 May 20;298(6):102057. doi: 10.1016/j.jbc.2022.102057

Figure 3.

Figure 3

MRAP2 inhibits the phosphorylation of GHSR1a.A, Western blot detection of MRAP2 in lysates of CHO-T-REx cells transfected with empty vector or with the bigenic HSVTK-3HA-GHSR1a-(CMV)/TetO2-MRAP2 construct in the presence or absence of tetracycline. B, ghrelin-stimulated IP1 accumulation assay in cells transfected with the bigenic construct in the presence or absence of tetracycline. C, 32P phosphoimaging and Western blot detection of GHSR1a following immunoprecipitation from cells transfected with HSVTK-3HA-GHSR1a-(CMV)/TetO2-MRAP2 without tetracycline and treated with ghrelin for the indicated time. D, 32P phosphoimaging and Western blot detection of GHSR1a following immunoprecipitation from cells transfected with HSVTK-3HA-GHSR1a-(CMV)/TetO2-MRAP2 with tetracycline and treated with ghrelin for the indicated time. E and F, densitometry analysis of GHSR1a phosphorylation in cells treated without (E) or with (F) tetracycline. Data are the mean ± SEM of three independent experiments. ∗ p < 0.05. CHO, Chinese hamster ovary; CMV, cytomegalovirus; GHSR1a, growth hormone secretagogue receptor 1a; IP, inositol triphosphate.