Fig. 6.

Influences of fullerenol or GSH on the cell viabilities and proliferation of hCECs exposed to UVB. A₁-A₄) Morphological features of hCECs under phase-contrast microscopy, showing morphological changes following 2h exposure to UVB and treated with 25 μg/ml fullerenol and 6.8 μg/ml GSH (the same molar concentration) for 24 h. B₁–B₄) Representative images of immunofluorescence for Ki67 (red) and DAPI (blue) in different groups: B₁) Control group. B₂) hCECs exposed to UVB for 2 h. B₃) hCECs treated with GSH for 24 h. B₄) hCECs treated with fullerenol for 24 h. C) Cell counting kit (CCK-8) detected cell viability of hCECs treated with fullerenol and GSH for 24 h respectively after UVB radiation damage G) Comparison of the number of Ki67-positive cells in different groups. The above detections were implemented in three independent experiments. Data were expressed as mean ± SEM from three independent experiments. *P < 0.1, **P < 0.01, ****P < 0.0001 using one-way ANOVA and post-hoc Tukey's test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)