Fig. 8.

Influences of fullerenol or GSH on the DNA oxidative damage and antioxidant capacity of hCECs exposed to UVB. A-D) Representative images of immunofluorescence for Nrf2 (red) and DAPI (blue) in different groups: A₁-A₃) Control group. B₁–B₃) UVB group. C₁–C₃) UVB + GSH group. D₁-D₃) UVB + Fullerenol group. E) Quantitative analysis of the number of Nrf2-positive cells of different groups. F–I) Detection of antioxidant protein HO-1 with immuno-fluorescence staining following exposure to UVB and the effect of GSH or fullerenol: F₁–F₃) Control group. G₁-G₃) UVB group. H₁–H₃) UVB + GSH group. I₁–I₃) UVB + Fullerenol group. J) Quantitative analysis of the number of HO-1-positive cells in different groups. Data were expressed as the mean ± SEM from three independent experiments. *P < 0.1, **P < 0.01, ***P < 0.001, ****P < 0.0001 using one-way ANOVA and post-hoc Tukey's test. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)