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. Author manuscript; available in PMC: 2022 Jun 13.
Published in final edited form as: FASEB J. 2021 Apr;35(4):e21426. doi: 10.1096/fj.202002464R

FIGURE 1.

FIGURE 1

Mfn2 expression is upregulated during muscle regeneration and myoblast differentiation. A, Mfn2 immunofluorescence in of uninjured and injured TA muscle sections at 3, 5, 7, and 21 days post-injury (dpi). Scale bar: 20 μm. B, Mfn2 immunofluorescence in primary myoblasts in growth medium (Day 0) and after differentiated for 1–4 days. Scale bar: 20 μm. C, qRT-PCR analysis of relative mRNA levels of Mfn2 and Myog genes (n = 3 each group). Data are shown as mean ± SEM (t test: **P < .01, ***P < .001). D, Western blots showing relative levels of Mfn2 and myogenesis-related proteins (Pax7, MyoD, and MF20) at various stages of myoblast differentiation