Fig. 5.

Anesthetic pharmacodynamics gradually normalize after termination of starvation-selection. (A) SS flies became susceptible to isoflurane preconditioning after the 8^th generation. (B) Postexposure toxicity of sevoflurane disappears in SS flies after the 8^th generation. Gray bars indicate the MI₂₄ without anesthetic exposure, purple bars indicate preconditioning with 15 minutes of 2% isoflurane, and yellow bars indicate postexposure to 15 minutes of 3.5% sevoflurane. SS gen 5–8 and SS gen 9–10 indicate the number of generations that the flies reproduced on cornmeal-molasses food. FC are fed control, i.e., lean flies. Note: the MI₂₄ of SS flies remains higher than that of FC flies. + indicates the mean, the horizontal line indicates the median, the box indicates 25^th to 75^th percentile, whiskers extend to the minimal and maximal values, and dots indicate individual replicates.