Skip to main content
. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: J Am Chem Soc. 2022 May 20;144(21):9302–9311. doi: 10.1021/jacs.2c00922

Figure 2.

Figure 2.

Impact of CD22L conjugated to anti-IgD on activation of murine B cells. Splenocytes from wildtype (WT; A) or CD22 knockout (CD22KO; B) mice on the C57BL/6J background were loaded with the fluorescent intracellular calcium indicator Indo-I AM, prior to staining with antibodies to analyze calcium flux in the B cells (B220+CD5−). Washed cells were resuspended in media containing CaCl2 and warmed to 37 °C prior to being stimulated with PBS or 10μg/ml of anti-IgD, (3) anti-IgD-N3, (5b) or anti-IgD-CD22L and analyzed by flow cytometry.