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. 2022 May 30;13:859381. doi: 10.3389/fendo.2022.859381

Figure 3.

Figure 3

β-Catenin, cyclin D1, P16, and Ki-67 expression in CP. (A–C) β-Catenin immunostaining score: (A) normal membranous staining in stratified squamous epithelial cells in PCP, (B) moderate and strong nuclear signal in 10%–50% and (C) >50% of neoplastic cells in two ACP cases, respectively. The signal was more represented in cells forming “whorled epithelium” structures. (D–F) Cyclin D1 immunostaining score: low nuclear signal observed in <10% of neoplastic cells (D, a case of PCP); moderate signal observed in 10%–50% of cells (E, a case of PCP); and strong reactivity in >50% of neoplastic cells (F, a case of ACP). (G–I) P16 immunostaining score: (G) signal with low intensity observed in <10% of neoplastic cells in PCP and (H) moderate intensity observed in 10%–50% of neoplastic cells in PCP; and (I) moderate and strong and diffuse (51%–80% of neoplastic cells) immunoreactivity in ACP. (J–L) Ki-67 labeling index: low proliferative index Ki-67 (<5%), and “whorled epithelium” structure (*) (J) extremely low Ki-67 index, (K) high Ki-67 index, and (I) Ki-67 index calculated as 15% in a case of PCP. All pictures were captured at 200× magnification.