Table 2.

Two-way ANOVA analysis of strain and time difference contribution to body weight, body fat/lean mass, food intake, energy expenditure, respiratory quotient, and motor activity in male and female control and TRPC6 KO mice

Measurements/Sex	Strain	Time	Strain × Time
Body weight
Male	P = 0.0002; F = 20.73	P < 0.0001; F = 104.10	P < 0.0001; F = 8.17
Female	P < 0.0001; F = 87.24	P < 0.0001; F = 71.82	P < 0.0001; F = 4.01
Body fat mass
Male	P < 0.0001; F = 32.55	P < 0.0001; F = 88.27	P < 0.0001; F = 5.06
Female	P < 0.0001; F = 35.62	P < 0.0001; F = 23.10	NS; F = 1.63
Body lean mass
Male	NS; F = 2.06	P < 0.0001; F = 49.60	P < 0.0001; F = 23.10
Female	P < 0.0001; F = 37.37	P < 0.0001; F = 38.27	P = 0.0239; F = 2.33
Food intake
Male	P < 0.0001; F = 26.58	P < 0.0001; F = 20.73	P = 0.0325; F = 2.07
Female	P < 0.0001; F = 88.32	P < 0.0001; F = 22.56	P < 0.0001; F = 8.88
Energy expenditure
Male	P < 0.0001; F = 36.76	NS; F = 1.17	NS; F = 0.41
Female	P = 0.0017; F = 15.06	P < 0.0001; F = 31.09	P < 0.0001; F = 4.43
Respiratory quotient
Male	P < 0.0001; F = 56.63	P < 0.0001; F = 6.78	NS; F = 0.53
Female	P < 0.0001; F = 139.40	P < 0.0001; F = 18.56	NS; F = 1.42
Motor activity
Male	P = 0.0007; F = 12.18	P < 0.0001; F = 20.37	NS; F = 0.63
Female	NS; F = 0.36	P < 0.0001; F = 43.48	NS; F = 0.97

TRPC6, transient receptor potential cation channel 6; KO, knockout; NS, not significant.