Table 3.

Three-way ANOVA analysis indicating how time, strain, and sex affected body weight, body fat/lean mass, food intake, energy expenditure, respiratory quotient, and motor activity in male and female control and TRPC6 KO mice

Measurements/Factors	Time	Strain	Sex	Time × Strain	Time × Sex	Time × Strain × Sex
Body weight	P < 0.0001; F = 160.60	P < 0.0001; F = 70.04	P < 0.0001; F = 106.20	P < 0.0001; F = 9.97	P < 0.0001; F = 9.11	NS; F = 1.18
Body fat mass	P < 0.0001; F = 80.80	P < 0.0001; F = 68.13	P = 0.0446; F = 4.46	P = 0.0315; F = 2.16	P < 0.0001; F = 7.06	P = 0.0017; F = 3.25
Body lean mass	P < 0.0001; F = 87.59	P < 0.0005; F = 15.65	P < 0.0001; F = 163.20	P < 0.0001; F = 17.31	P < 0.0001; F = 5.40	P < 0.0001; F = 9.02
Food intake	P < 0.0001; F = 41.82	P < 0.0001; F = 78.20	P < 0.0001; F = 34.24	P < 0.0001; F = 9.49	NS; F = 1.25	NS; F = 1.28
Energy expenditure	P < 0.0001; F = 47.33	P = 0.0014; F = 12.82	P < 0.0001; F = 30.06	P < 0.0001; F = 11.00	P < 0.0001; F = 4.52	NS; F = 0.58
Respiratory quotient	P < 0.0001; F = 38.80	P < 0.0001; F = 33.82	P = 0.0485; F = 4.30	NS; F = 1.04	P = 0.0173; F = 2.30	P = 0.0271; F = 2.14
Motor activity	P < 0.0001; F = 73.35	NS; F = 2.06	NS; F = 1.07	NS; F = 0.89	P = 0.0260; F = 2.16	NS; F = 1.23

TRPC6, transient receptor potential cation channel 6; KO, knockout; NS, not significant.