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Sensitivity of primer pair ECA75F-ECR619R in the optimized PCR for amplification of E. coli 16S rRNA DNA template. (A) Agarose gel separation of PCR mixtures; (B) Southern hybridization of the gel in panel A to EUB338. Lanes: 1, 100-bp DNA ladder; 2, no template; 3 to 12, 100 pg, 50 pg, 10 pg, 5 pg, 1 pg, 500 fg, 100 fg, 50 fg, 10 fg, and 5 fg of E. coli genomic DNA.
