FIGURE 4.

Venn diagram constructed using the OrthoVenn online service (Wang et al., 2015) showing the distribution of shared gene families (orthologous clusters) among different Clavibacter species (a). Pairwise alignment among the chromosomal DNA of Clavibacter sepedonicus ATCC 33113^T and three closely related Clavibacter species using MAUVE software (Darling et al., 2010) using default parameters (match seed weight = 15). Colours show conserved and highly related genomic regions (locally collinear blocks). Blocks shifted below the centre line indicate segments that align in the reverse orientation as inversions relative to reference strain ATCC 33113^T. Each commonly coloured region is a locally collinear block, which is a region without rearrangement of the homologous backbone sequence. Lines between two genomes trace each orthologous locally collinear block (b). Phenotypic and genotypic differences between the pathogenic and nonpathogenic strains of C. sepedonicus on potato plants and nonhost (tobacco) plants (c)