FIGURE 2.

ChpG is required for elicitation of hypersensitive response (HR) in eggplant. Black Queen eggplant and Moneymaker tomato leaves were infiltrated (10⁸ cfu/ml for a and b, 10⁴ cfu/ml for c) with Clavibacter michiganensis wild‐type CMM101 (Cm), CMM101 chpG inactivation mutant (CmΩchpG), and CmΩchpG carrying pHN216:ChpG (CmΩchpG + ChpG). (a) Leaves were photographed 72 h after infiltration. (b) Cell death was quantified by ion leakage at 24 and 36 h postinfiltration. Lower and upper quartiles are marked at the margins of the boxes. Central lines and “o” represent medians and data points of at least 15 (for eggplant) or 10 (for tomato) biological repeats collected from two (tomato at 24 h) or three independent experiments. * indicates significant difference (Mann–Whitney U test, p ≤ 0.05) from Cm. (c) Leaf apoplast bacterial populations of the indicated Cm strains were quantified at 0, 3, and 6 days postinoculation (dpi). Lower and upper quartiles are marked at the margins of the boxes. Central lines and “o” represent medians and data points of 15 (for eggplant) or 10 (for tomato) biological repeats collected from two (tomato) or three (eggplant) independent experiments. * indicates significant difference (Mann–Whitney U test, p ≤ 0.05) from Cm