FIG. 3.

Percentage of coverage by L. monocytogenes biofilm on stainless steel over a 4-h period, determined by staining with acridine orange. Fifteen microscopic fields of each coupon were analyzed. The area covered by the bacteria on each field was compared to the total area of a microscopic field as an absolute value (100%). ■ and ●, wild type incubated at 22 and 37°C, respectively; □ and ○, nonflagellated mutant incubated at 22 and 37°C, respectively. Error bars show standard deviations of values from triplicate coupons.