Less collagen was recovered in cell culture media in the scaffolds where a CS-SILY molecule was added. Collagen from media aliquots in which cell-hydrogel constructs, with or without added CS or CS-SILY molecules, were cultured. Medium aliquots were pooled together from each week and analyzed at 7, 14, 21, or 28 days. Collagen was measured using a Sircol dye assay kit. Values are expressed as mean ± standard deviation (n = 3). An ANOVA and Tukey’s post hoc tests were performed. Data that share the same letter do not have statistically significant differences (p > 0.05) whereas data that do not share the same letter have statistically significantly differences (p < 0.05).