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. Author manuscript; available in PMC: 2022 Jun 13.
Published in final edited form as: ACS Sens. 2022 Mar 3;7(3):900–911. doi: 10.1021/acssensors.2c00024

Table 2.

Summary of the reported CRISPR-based diagnostics with LOD and CRISPR reaction time available.

Pathogen Target Effector Readout System Amplification Amplification time (min) CRISPR reaction time (min) LOD (aM) FOM (aM.min) Ref.
Ensemble without amplification
African Swine Fever DNA LbCas12a Fluorescence None None 480 1e6 4.8e8 110
African Swine Fever DNA LbCas12a Fluorescence None None 1440 1e5 1.4e8 110
Pseudorabies virus DNA LbCas12a Fluorescence None None 15 1e8 1.5e9 8
Liver cancer DNA LbCas12a Colorimetric None None 60 2e8 1.2e10 15
HPV RNA LbCas12a Electrochemical None None 60 3e7 1.8e9 105
Zika virus RNA LwCas13a Fluorescence None None 60 5e5 3e7 9
SARS-CoV-2 RNA LbuCas13a Fluorescence None None 120 1.6e4 1.9e6 69
Synthesized target RNA LbuCas13a Fluorescence None None 120 1e6 1.2e8 104
Synthesized target RNA LbuCas13a Fluorescence None None 20 3.7e9 7.4e10 83
HPV DNA LbCas12a Electrochemical None None 60 5e7 3e9 20
miR-19b and miR-20a mRNA LwaCas13a Electrochemical None None 15 1e7 1.5e8 14
DENV-4 DNA AsCas12a Electrochemical None None 120 1e5 1.2e7 94
BRCA-1 DNA AsCas12a Fluorescence None None 30 1e3 3e4 93
HPV DNA LbCas12a Fluorescence None None 60 1e4 6e5 92
Bacillus anthracis gene DNA LbCas12a Fluorescence None None 15 1e7 1.5e8 91
Synthesized target DNA LbCas12a
 Fluorescence None None 60 1e5 6e6 90
Ensemble with amplification
Citrus greening disease DNA LbCas12a Fluorescence LAMP 40 5 16.6 83 112
African Swine Fever (ASF) DNA LbCas12a Fluorescence LAMP 40 20 3.6 72 111
HPV DNA LbCas12a Fluorescence RPA 15 60 16.6 1e3 109
ASF DNA LbCas12a Fluorescence RPA 30 60 10 600 108
SARS-CoV-2 RNA LbCas12a Fluorescence RPA 30 30 16.6 498 116
HPV DNA LbCas12a Fluorescence RPA 10 60 10 600 52
Pseudorabies virus DNA LbCas12a Fluorescence PCR 45 15 10 150 8
SARS-CoV-2 RNA LbCas12a Fluorescence RPA (one pot) None 40 80.3 3.2e3 25
P.aeruginosa DNA LbCas12a Colorimetric LAMP 15 30 3.4 102 107
HPV DNA LbCas12a Colorimetric PCR 50 30 240 7.2e3 106
Ebola virus RNA LbCas12a Fluorescence RPA 40 240 10 2.4e3 46
Synthesized target RNA AacCas12b Fluorescence LAMP 30 30 10 300 16
SARS-CoV-2 RNA AacCas12b Fluorescence RAA 30 30 16.6 498 26
Zika virus RNA LwCas13a Fluorescence RPA 120 60 2 120 9
Zika Virus RNA LbuCas13a Fluorescence RPA 20 60 6 360 19
Cytomegalovirus DNA LwCas13a Fluorescence RPA 50 180 0.6 108 103
White Spot Syndrome RNA Cas13a Colorimetric RPA 40 180 1.6 288 102
Various tumor cells mRNA LbuCas13a Electrochemical EXPAR 30 30 1e3 3e4 21
SARS-CoV-2 RNA AsCas12a Nanopore PCR 30 30 22.5 675 45
SARS-CoV-2 RNA LbCas12a Fluorescence LAMP 30 10 16.6 166 23
SARS-CoV-2 RNA AsCas12a Fluorescence LAMP 30 30 8.3 249 24
SARS-CoV-2 RNA LwaCas13a Fluorescence RPA 20 60 16.6 996 28
SARS-CoV-2 RNA LbCas12a Fluorescence LAMP 20 15 16.6 249 101
HPV DNA AaCas12b Fluorescence RPA 10 180 1 180 100
SARS-CoV-2 RNA AapCas12b Fluorescence LAMP (one pot) None 60 3.3 198 48
Different Viruses RNA LwaCas13a Fluorescence PCR or RPA 20 180 0.9 162 99
SARS-CoV-2 RNA LbCas12a Colorimetric RPA 30 20 8.3 166 98
Listeria monocytogenes DNA LbCas12a Electrochemical RAA 30 90 0.68 61.2 97
SARS-CoV-2 RNA LbCas12a Fluorescence RPA 30 10 16.6 166 96
SARS-CoV-2 RNA LwaCas13a Fluorescence PCR 22 30 332 1e4 27
SARS-CoV-2 RNA LbCas12a Colorimetric RPA 20 60 1.6 96 95
SARS-CoV-2 RNA LbCas12a Fluorescence RPA 15 25 83 2.1e3 89
Staphylococcus aureus DNA LbCas12a Colorimetric RAA 20 30 1 30 88
SARS-CoV-2 RNA LbCas12a Fluorescence RPA (one pot) None 60 2 120 113
SARS-CoV-2 RNA LbCas12a
 Fluorescence LAMP 10 25 6.5 162.5 114
SARS-CoV-2 RNA LbCas12a
 Fluorescence LAMP 40 10 26 260 115
Ensemble using multiple crRNA
SARS-CoV-2 RNA LbuCas13a Fluorescence None None 120 166 2e4 69
Synthesized target DNA LbCas12a Fluorescence None None 30 310 9.3e3 117
Digital without amplification
ASF DNA LbCas12a Fluorescence None None 60 30 1.8e3 65
SARS-CoV-2 RNA LbuCas13a Fluorescence None None 60 10 600 74
SARS-CoV-2 RNA LbuCas13a Fluorescence None None 15 8.3 124.5 72
Digital with amplification
SARS-CoV-2 RNA LbCas12a Fluorescence RPA (one pot) None 60 1.5 90 86
SARS-CoV-2 RNA Cas12a Fluorescence RPA (one pot) None 60 1.6 96 85
SARS-CoV-2 RNA LbCas12a Fluorescence DAMP (one pot) None 50 8.3 415 39
SARS-CoV-2 RNA AapCas12b Fluorescence LAMP (one pot) None 120 23 2.7e3 118
Digital using multiple crRNA
SARS-CoV-2 RNA LbuCas13a Fluorescence None None 15 1.6 24 72