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. Author manuscript; available in PMC: 2022 Jun 13.
Published in final edited form as: Cytometry A. 2008 May;73(5):400–410. doi: 10.1002/cyto.a.20555

Figure 3.

Figure 3.

Fluorescence minus one (FMO) controls. To unequivocally identify viable memory and effector T cell subpopulations, all gates were controlled using a stain that lacks just one of the fluorescent markers of interest. (A) Here, 5% contour plots with outliers show how a FMO control (left panel) is used to identify live cells in the fully stained sample (right panel). From there subsequent gating identifies CD3+ and then CD4 and CD8 single-stained cells. (B) Similarly, gating strategies to identify T cell subsets based on CD45RA and CCR7 on CD4 (top) and CD8 (bottom) staining is done comparing each stain to its respective FMO control.