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. 2022 Jun 1;119(23):e2202799119. doi: 10.1073/pnas.2202799119

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Copyright © 2022 the Author(s). Published by PNAS

This open access article is distributed under Creative Commons Attribution License 4.0 (CC BY).

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Fig. 6. — Mapping CLMS data derived from DNA-free Smc5/6 onto the structure of DNA-bound Smc5/6. (A) Interprotein CL pairs between Smc5 and Smc6 indicate arm opening. (B) Interprotein CL pairs connecting Nse1-3-4 with Smc5 or Smc6 suggest that Nse3 moves away from the Smc5 head/neck regions to be positioned above DNA. (C) Interprotein CL pairs between Nse1, Nse3 and Nse4 suggest that the Nse1-WHB and Nse3-WHA domain move away from the Nse4-WHD and adjacent linker 2 (Lk2) region. (D) Intra-Nse4 CL pairs are indicative of large conformational changes within Nse4 midregion. (E) Intra-subunit CL pairs for Smc5 and 6. (F) Intra-subunit CL pairs for Nse1 and Nse3. (G) A model of Smc5/6 conformational changes upon DNA binding. The arrows indicate conformational changes in Smc5/6 upon encircling of DNA.