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. 2022 Jun 13;18(6):e1010229. doi: 10.1371/journal.pgen.1010229

Fig 4. KLF15 promoted autophagy and apoptosis as detected by autophagic flux and CASP3 activity.

Fig 4

A and B. The autophagic flux were detected in HaEpi cells when Klf15 was knocked down, dsYFP was used as the control. 3-MA (10 μM) is an autophagosome formation inhibitor; the yellow bars represented 20 μm. The yellow arrows represented autophagosome puncta. The blue arrows represented autolysosome puncta. Ai and Bi. Counted the number of autophagosome puncta and autolysosome puncta in successfully transfected with pIEx-GFP-RFP-LC3 cells. C. After knocked down Klf15 in HaEpi cells, examination of apoptosis by the addition of active CASP3, Ac-DEVD-CHO is a CASP3 inhibitor, the bars represented 50 μm. Ci. Quantification apoptotic cells in total from C.