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. 2022 Jun 13;18(6):e1010229. doi: 10.1371/journal.pgen.1010229

Fig 5. KLF15 promoted autophagy through upregulating Atg8.

Fig 5

A. Nuclear proteins from KLF15-RFP-His overexpressed cells were extracted for EMSA. B. KLF bs on the Atg8 promoter bound to KLF15 detected by EMSA assay. WT and MT represent KLF bs probe and KLF bs mutant probe, respectively. C. ChIP assay showing 20E promoted Atg8 expression via KLF15 binding to KLF bs. Primer CACCC targeting KLF bs. Primer Atg8 targeting Atg8 ORF. D. Changes in the expression levels of Atg8 and Casp3 after Klf15 knockdown.