Figure 4.

DNP-enhanced 1D ZF-TEDOR experiments of array samples prepared with a) ¹³C,¹⁵N-labeled H4, b) ¹³C-labeled H4, ¹⁵N-labeled H3, and c) ¹⁵N-labeled H3. The spectrum in a) was acquired with 16 scans, while the spectra in b) and c) were acquired with 4096 scans. Samples in a) and c) were prepared with ¹³C-depleted glycerol, while the sample in b) was prepared with natural abundance glycerol, causing the negative peaks in the spectrum. Samples contained ~ 4.5, 4.5 and 2.2 mg of nucleosome arrays, respectively. τ_mix = TEDOR mixing time.