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Kinetic parameters of wild-type phospholipase A1 and mutant phospholipase A1 variants with phosphatidylcholine at pH 8.0 and 37°Ca
| Enzyme | Km (μM) | kcat (s−1) | kcat/Km (s−1 · μM) |
|---|---|---|---|
| Wild type | 114.5 | 11.23 | 0.098 |
| TA3 | 105.1 | 10.92 | 0.104 |
| TA13 | 120.4 | 13.67 | 0.114 |
All values are averages based on values from at least duplicate assays.
