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. 2022 Jun 13;12(6):e848. doi: 10.1002/ctm2.848

FIGURE 3.

FIGURE 3

Overexpression of YTHDF2 significantly promotes ICC cell proliferation, inhibits apoptosis and decreases cell cycle arrest in G0/G1 stage. (A) The mRNA expression of YTHDF2 in HuCC‐T1 and HCCC‐9810 cells transfected with control or YTHDF2 vector were measured by qRT‐PCR. (B) The protein expression of YTHDF2 in HuCC‐T1 and HCCC‐9810 cells transfected with control or YTHDF2 vector were measured by western blotting. (C) Cell viability of HuCC‐T1 and HCCC‐9810 cells transfected with control or YTHDF2 vector. (D) Colony formation analysis of HuCC‐T1 and HCCC‐9810 cells transfected with control or YTHDF2 vector. (E) Cell cycle analysis of HuCC‐T1 and HCCC‐9810 cells transfected with control or YTHDF2 vector. (F) Apoptosis analysis of HuCC‐T1 and HCCC‐9810 cells transfected with control or YTHDF2 vector. (G) Immunoblotting to measure YTHDF2, CDK2, cyclinD3, cyclinE1 and CDK4 protein levels in HuCC‐T1 and HCCC‐9810 cells transfected with control or YTHDF2 vector. The data are presented as mean ± SD and compared by t‐test. *p < .05; **p < .01; *** p < .001