Figure 4. Deletion of AAT2 increases oxidative stress sensitivity.

(A) Spotting assay of BY4741 parent and aat2Δ strains on synthetic complete dextrose (SC) medium in the presence of varying concentrations of hydrogen peroxide (H₂O₂). Each spot is a tenfold dilution of the previous one. (B) Representative polysome profiles from unstressed and H₂O₂-treated cultures. P/M: mean polysome-to-monosome ratio (n = 2–3).

Figure 4—figure supplement 1. Metabolic functions are enriched among RNA-binding proteins (RBPs) identified in polysomal fractions.

Deletion strains for multiple candidate RBPs identified by polysomal proteomics were tested for sensitivity to oxidative stress. The slf1Δ strain was previously observed to be hypersensitive to oxidative stress so was used as a control (Kershaw et al., 2015). (A) Spotting assay on synthetic complete dextrose (SC) medium in the presence of varying concentrations of hydrogen peroxide (H₂O₂). Each spot is a tenfold dilution of the previous one. (B) Representative polysome profiles from unstressed and H₂O₂-treated cultures (n = 2–3).