Figure 5. Deletion of AAT2 enhances Gcn2 activity during oxidative stress.

(A) Left: representative western blots showing eIF2α phosphorylation in unstressed and hydrogen peroxide (H₂O₂)-treated cultures. Right: bands were quantified using LI-COR Image Studio and the eIF2α-P/eIF2α ratio was calculated. Error bars show standard deviation (SD) (n = 3). (B) Top: GCN4-lacZ reporter constructs used to test the translational activation of GCN4. Solid boxes – lacZ ORF, open boxes – GCN4 upstream ORFs, crosses – removed GCN4 uORFs. Bottom: β-galactosidase activity in strains transformed with GCN4-lacZ reporter plasmids (n = 3–10). Error bars show SD. U – unstressed, H – +0.45 mM H₂O₂. The t-test was used to compare the strains: ns – not significant (p > 0.05), *p < 0.05, **p < 0.01.