Figure 6. Aat2 binds to 60S ribosomes.

(A) Representative polysome profile and western blot of an unstressed cell extract from the Aat2-TAP strain run on a 15–50% sucrose gradient. (B) Ribosome pellets from unstressed extracts were isolated using sucrose cushions, then either left untreated or treated with RNase I prior to polysome profiling. (C) Representative polysome profile and western blot from an unstressed cell extract treated with 50 mM ethylenediaminetetraacetic acid (EDTA) and run on a 10–25% sucrose gradient to separate the ribosomal subunits.