|
Antibody
|
α-Rps3 (rabbit polyclonal) |
PMID:11278502
|
|
Diluted in 5% milk-TBST (1:5000) |
|
Antibody
|
α-Rpl35 (rabbit polyclonal) |
PMID:11278502
|
|
Diluted in 5% milk-TBST (1:5000) |
|
Antibody
|
α-Pab1 (mouse monoclonal) |
EnCor Biotechnology |
RRID:AB_2572370
|
Diluted in 5% milk-TBST (1:4000) |
|
Antibody
|
α-Scp160 (rabbit polyclonal) |
PMID:11278502
|
|
Diluted in 5% milk-TBST (1:10,000) |
|
Antibody
|
α-protein A (rabbit polyclonal) |
Sigma-Aldrich P1291 |
RRID:AB_1079562
|
Diluted in 5% milk-TBST (1:1000) |
|
Antibody
|
α-Sui2 (chicken polyclonal) |
PMID:31086188
|
|
Diluted in 5% milk-TBST (1:1000) |
|
Antibody
|
α-phospho-Sui2 (rabbit polyclonal) |
Cell Signalling Technologies |
RRID:AB_330951
|
Diluted in 5% milk-TBST (1:1000) |
|
Antibody
|
IRDye 800CW Goat anti-Rabbit IgG, H + L (goat polyclonal) |
LI-COR Biosciences |
Cat. no.: 926-32211 |
Diluted in 5% milk-TBST (1:10,000) |
|
Antibody
|
IRDye 680RD Goat anti-Mouse IgG, H + L (goat polyclonal) |
LI-COR Biosciences |
Cat. no.: 926-68070 |
Diluted in 5% milk-TBST (1:10,000) |
|
Antibody
|
IRDye 680RD Donkey anti-Chicken Ig, H + L (donkey polyclonal) |
LI-COR Biosciences |
Cat. no.: 926-68075 |
Diluted in 5% milk-TBST (1:10,000) |
|
Chemical compound, drug
|
Hydrogen peroxide solution |
Sigma |
Cat. no.: 95,321 |
Made 1/100 dilution in water before adding to cultures/media |
|
Chemical compound, drug
|
3-Amino-1,2,4-triazole |
Sigma |
Cat. no.: A8056 |
|
|
Peptide, recombinant protein
|
Ambion RNase I |
Thermo Fisher |
Cat. no.: AM2294 |
|
|
Peptide, recombinant protein
|
Sequencing Grade Modified Trypsin |
Promega |
Cat. no.: V5111 |
|
|
Commercial assay or kit
|
NuPAGE 4% to 12%, Bis-Tris, 1.0 mm, Mini Protein Gel, 12-well |
Life Technologies |
Cat. no.: NP0322 |
|
|
Strain, strain background (E. coli) |
XL10-Gold Ultracompetent Cells |
Agilent |
Cat. no.: 200,314 |
|
|
Strain, strain background (S. cerevisiae)
|
S288c |
Detailed in Supplementary file 1, Sheet 6 |
|
|
|
Software, algorithm
|
LI-COR Image Studio and LI-COR Image Studio Lite |
LI-COR Biosciences |
https://www.licor.com/bio/software
|
Version 5.2. LI-COR Image Studio Lite has since been discontinued. |
|
Software, algorithm
|
R |
R Core Team, 2019
|
https://www.r-project.org/
|
Version 3.6.2 |
|
Software, algorithm
|
MaxQuant |
Tyanova et al., 2016
|
https://www.maxquant.org/
|
Version 1.5.7.4 |
|
Software, algorithm
|
GNU Image Manipulation Program |
GIMP |
https://www.gimp.org/
|
Version 2.8.10 |
