Figure 3. Hnrnpa2b1 is upregulated in myogenic nuclei during skeletal muscle regeneration.

(A) Hnrnpa2b1 immunoreactivity in uninjured (UI), 5, 10, and 30 days post injury (dpi) regenerating mouse muscle. All images represent n = 3 biological replicates; scale = 20 µM. (B) Nuclear Hnrnpa2b1 immunoreactivity in UI, 5, 10, and 28 dpi regenerating muscle. (C) Nuclear Hnrnpa2b1 immunoreactivity intensity in either myonuclei or non-myonuclei in UI, 5, 10, and 28 dpi regenerating muscle. See also Figure 3—figure supplement 1.

Figure 3—figure supplement 1. Related to Figure 3.

(A) Secondary-only immunoreactivity controls of muscle sections for all time points assessed by immunofluorescence (IF) in Figure 1A. (B) Quantified Western blot analysis of Hnrnpa2b1 protein expression from whole uninjured (UI) and 5 days post injury (dpi) regenerating muscle (one-tailed Student’s t-test). (C) Hnrnpa2b1 nuclear intensity in Pax7-positive myonuclei in UI, 5, 10, and 28 dpi regenerating muscle. (D) Scatter plot and R² values for expression changes in myonuclear Pax7 and Hnrnpa2b1 in UI, 5, 10, and 28 dpi regenerating muscle. (E) Immunoreactivity in 5 dpi regenerating muscle for Tardbp, myosin heavy chain (Myhc), and Pax7. (F) Scatter plot and R² values for Tardbp expression changes in Pax7 myonuclei in UI and 5 dpi. (G) Tardbp nuclear protein in UI, 5 dpi , and 10 dpi regenerating muscle. All images represent n = 3 biological replicates; scale = 20 µM. All quantified data represent mean ± SD, two-tailed Student’s t-test p-value: *<0.05, **<0.01, ***<0.001, ****<0.0001 unless otherwise stated.