Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Arabidopsis thaliana) | HSL3 | ARAPORT11 | AT5G25930, Q9XGZ2_ARATH | |
| Gene (Arabidopsis thaliana) | CTNIP1 | ARAPORT11 | AT1G06135, Q8LCX3_ARATH | |
| Gene (Arabidopsis thaliana) | CTNIP2 | ARAPORT11 | AT1G06137, F4IBZ9_ARATH | |
| Gene (Arabidopsis thaliana) | CTNIP3 | ARAPORT11 | AT2G31335, Q1G3B9_ARATH | |
| Gene (Arabidopsis thaliana) | CTNIP4 | ARAPORT11 | AT2G31345, Q8L9Z1_ARATH | |
| Gene (Arabidopsis thaliana) | CTNIP5 | ARAPORT11 | AT2G23123, A0A1I9LM80_ARATH | |
| Gene (Medicago truncatula) | MtHSL3 | Mt4.0 | G7J3I8_MEDTR, MTR_3g110450, MtrunA17_Chr3g0140551 | |
| Gene (Medicago truncatula) | MtCTNIP | Mt4.0 | G7I613_MEDTR, MTR_1g044470, MtrunA17_Chr1g0168241 | |
| Genetic reagent (Arabidopsis thaliana) | hsl3-1 | euNASC | salk_207895 | |
| Genetic reagent (Arabidopsis thaliana) | hsl3-2 | euNASC | wiscdslox450b04 | |
| Genetic reagent (Arabidopsis thaliana) | bak1-4/pBAK1::BAK1-GFP | https://doi.org/10.1105/tpc.111.090779 | bak1-4/pBAK1::BAK1-GFP | |
| Genetic reagent (Arabidopsis thaliana) | bak1-5 | https://doi.org/10.1371/journal.pgen.1002046 | bak1-5 | |
| Genetic reagent (Arabidopsis thaliana) | p35S::AEQUORIN | https://doi.org/10.1038/352524a0 | ||
| Genetic reagent (Nicotiana benthamiana) | p35S::AEQUORIN | https://doi.org/10.1104/pp.110.171249 | ||
| Genetic reagent (Arabidopsis thaliana) | 35S::HSL3-GFP | This paper | Figure 3 and Materials and methods | |
| Genetic reagent (Arabidopsis thaliana) | pHSL3::BIR3-HSL3-FLAG | This paper | Chimera created using the method from https://doi.org/10.1105/tpc.20.00138 |
|
| Cell line (Trichoplusia ni) | Tnao38 | https://doi.org/10.1186/1472-6750-12-12 | Cell line maintained in J Santiago lab | |
| Antibody | Anti-BAK1 (rabbit polyclonal) | https://doi.org/10.1105/tpc.111.084301 | WB (1:2000) | |
| Antibody | Anti-GFP (HRP-conjugated mouse monoclonal) | Santa Cruz | sc-9996 | WB (1:5000) |
| Antibody | Anti-pMAPK (rabbit polyclonal) | Cell Signaling | p44/42 MAPK (Erk1/2) antibody #9,102 | WB (1:4000) |
| Recombinant DNA reagent | 35S::HSL3-GFP (plasmid) | This paper | Figure 3 | |
| Recombinant DNA reagent | pHSL3::BIR3-HSL3-FLAG (plasmid) | This paper | Used to generate transgenic plants in Figure 3 | |
| Recombinant DNA reagent | pHSL3::LTI6B-Citrine (plasmid) | This paper | Used to generate transgenic plants in Figure 3—figure supplement 4 | |
| Recombinant DNA reagent | pHSL3::BIR3F146A,R170A -HSL3-Citrine (plasmid) | This paper | Used to generate transgenic plants in Figure 3—figure supplement 4 | |
| Recombinant DNA reagent | pHSL3::BIR3-HSL3-CITRINE (plasmid) | This paper | Used to generate transgenic plants in Figure 3—figure supplement 4 | |
| Commercial assay or kit | GFP-Trap | Chromotek | Cat. #: gta-20 | |
| Software, algorithm | GraphPad | GraphPad software |