TABLE 2.
Frequency of phage types occurring after plaquing φ31 on Per31+ NCK203 and its Per31+ insertion derivatives, NCK203-A, NCK203-B, and NCK203-C
Per31r strain harboring pTRK361 | Frequencies of recombinant phage typesa
|
Total no. of plaques examined | ||||
---|---|---|---|---|---|---|
φ31b | φ31.1 | φ31.2 | φ31.7 | φ31.8 | ||
NCK203 | 1c | 30 | 9 | 5 | 3 | 48 |
NCK203-A | 8 | 8 | 0 | 4 | 0 | 20 |
NCK203-B | 4 | 0 | 13 | 0 | 7 | 24 |
NCK203-D | 1 | 0 | 7 | 4 | 3 | 15 |
Each occurrence of a phage type was based on the HindIII or EcoRI band pattern of DNA from an isolated single plaque. The numbers are total numbers based on results obtained by using two or three separately propagated phage lysates.
The EOP for φ31 on all Per31+ strains was 10−6 to 10−7.
Per31r variant of φ31 which had the same restriction fragment pattern as φ31.