FIG. 2.

Incorporation of monomers into PHA by P. putida KT2442 (■) and by aceA knockout mutant P. putida KT217 (□) grown on substrate mixtures containing gluconate and heptanoate. Cells were cultivated by using the following substrate mixtures in 0.1 N E2 minimal medium: 42 mM gluconate and 2 mM heptanoate (gluconate/heptanoate ratio, 21), 32 mM gluconate and 6 mM heptanoate (gluconate/heptanoate ratio, 5), 23 mM gluconate and 10 mM heptanoate (gluconate/heptanoate ratio, 2), and 5 mM gluconate and 18 mM heptanoate (gluconate/heptanoate ratio, 0.3). Samples were harvested after 48 h of cultivation, lyophilized, and analyzed by gas chromatography. (A) Incorporation of C₇ monomers (3-hydroxyheptanoate) into mcl PHA relative to total cell dry weight (cdw). (B) Total mass of Ceven monomers (3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, and 3-hydroxydodecanoate–3-hydroxydodecenoate) incorporated into PHA relative to total cell dry weight.