TABLE 1.
Specific activities of isocitrate lyase and isocitrate dehydrogenase in crude extracts of P. putida KT2442 and P. putida KT217a
| Substrate(s) | Length of cultivation (h) | Sp act (nmol min−1 mg−1)b
|
|||
|---|---|---|---|---|---|
| Isocitrate lyase
|
Isocitrate dehydrogenase
|
||||
| KT2442 | KT217 | KT2442 | KT217 | ||
| Gluconate (46 mM) | 24 | 43 | 2 | 569 | 182 |
| 48 | 35 | 2 | 498 | 109 | |
| Gluconate (23 mM) + heptanoate (10 mM) | 24 | 321 | 3 | 445 | 205 |
| 48 | 147 | 3 | 318 | 122 | |
a
Cells were cultivated in 0.1 N E2 minimal medium supplemented with gluconate or a mixture of gluconate and heptanoate. The cells were harvested at the end of the exponential phase (24 h) and in the late stationary phase (48 h).
b
Crude extracts were prepared and used in assays for isocitrate lyase and isocitrate dehydrogenase activities.