TABLE 2.
PHA contents and monomer compositions of P. putida KT2442 and KT217 cultivated on gluconatea
| Organism | Time (h) | Cell dry wt (g liter−1) | PHA content (g liter−1) | Maximal growth rate (h−1) | PHA content (%, wt/wt) | PHA monomer composition (mol%)b
|
||||
|---|---|---|---|---|---|---|---|---|---|---|
| C6 | C8 | C10 | C12 | C12:1 | ||||||
| KT2442 | 24 | 0.68 | 0.12 | 0.19 | 18.1 ± 2.1 | 0 | 12.9 | 74.1 | 7.1 | 5.9 |
| 48 | 0.57 | 0.20 | 37.9 ± 3.4 | 0.8 | 14.9 | 71.5 | 7.1 | 5.7 | ||
| KT217 | 24 | 0.7 | 0.18 | 0.25 | 27.4 ± 2.6 | 0.6 | 13.5 | 73.1 | 7.0 | 5.8 |
| 48 | 0.78 | 0.34 | 41.0 ± 4.0 | 0.9 | 15.2 | 70.9 | 7.2 | 5.8 | ||
a
Cells were cultivated in 0.1 N E2 minimal medium supplemented with 1% gluconate. At the end of the exponential phase (24 h) and during the late stationary phase (48 h) cells were harvested and lyophilized, and the PHA content and monomer composition were determined by gas chromatography. The values are averages based on the values obtained with triplicate samples in three independent experiments.
b
C6, 3-hydroxyhexanoate; C7, 3-hydroxyheptanoate; C8, 3-hydroxyoctanoate; C10, 3-hydroxydecanoate; C12, 3-hydroxydodecanoate; C12:1, 3-hydroxy-5-cis-dodecenoate.