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. Author manuscript; available in PMC: 2022 Jun 13.
Published in final edited form as: Br J Pharmacol. 2021 Feb 14;178(6):1426–1444. doi: 10.1111/bph.15352

Figure 5.

Figure 5.

Immunoblot analysis to detect dDAVP-induced phosphorylation on Camkk2. A. Camkk2 and pS511-Camkk2 immunoblot from dDAVP- and vehicle-treated mpkCCD cells. The gels were stained with Coomassie stain to check equal loading among different samples. B. Separated scatter plot showing the ratio of normalized immuno-reactivities (mean ± SEM in arbitrary units) of pS511-Camkk2 and Camkk2. Unpaired t-test was used to determine significant difference between dDAVP and vehicle-treated samples. dDAVP caused a significant increase in phosphorylation of S511 of Camkk2 in mpkCCD cells. n, experimental replicate for vasopressin stimulation experiment. *P < .05. A number of immunoblots using phospho-specific antibodies have been reported previously (https://esbl.nhlbi.nih.gov/ESBL/WB-data/).