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. Author manuscript; available in PMC: 2022 Jun 13.
Published in final edited form as: Br J Pharmacol. 2021 Feb 14;178(6):1426–1444. doi: 10.1111/bph.15352

Table 1.

Protein kinases with vasopressin-responsive phosphopeptides in mpkCCD renal epithelial cells

Gene Symbol Mod. Site(s) Annotation Peptide Sequence Centralized Sequence Log2 (dDAVP/Vehicle) −log10 (CV)
Sik2 S358 Serine/threonine-protein kinase SIK2 RPS*TIAEQTVAK GRQRRPS*TIAEQT 2.10 0.40
Nrbp2 S359 Nuclear receptor-binding protein 2 YS*EVSFLELDK PMQWRYS*EVSFLE 1.94 0.62
Eif2ak2 S110 Interferon-induced, double-stranded RNA-activated protein kinase LS*VNYEQCEPNSELPQR AQKKKLS*VNYEQC 1.80 0.47
Camkk2 S511 Calcium/calmodulin-dependent protein kinase kinase 2 SLS*APGNLLTK REERSLS*APGNLL 1.71 0.54
Map4k5 S433, S434 Mitogen-activated protein kinase kinase kinase kinase 5 RQS*S*PSCVPVAETSSSIGNGDGISK - 1.36 0.79
Map3k2 T337 Mitogen-activated protein kinase kinase kinase 2 RRGSDIDNPT*LTVTDISPPSR SDIDNPT*LTVTDI 1.13 0.42
Scyl2 S677 SCY1-like protein 2 RGS*LTLEEK GKQKRGS*LTLEEK 1.04 0.75
Map4k5 T400 Mitogen-activated protein kinase kinase kinase kinase 5 VNT*YPEDSLPDEEK PKPRVNT*YPEDSL 0.95 1.35
Cdk18 S66 Cyclin-dependent kinase 18 RFS*MEDLNK QNQRRFS*MEDLNK 0.88 0.42
Map3k2 S331 Mitogen-activated protein kinase kinase kinase 2 RRGS*DIDNPTLTVTDISPPSR RIRRRGS*DIDNPT 0.78 0.84
Map3k2 S331, S344, S347 Mitogen-activated protein kinase kinase kinase 2 RRGS*DIDNPTLTVTDIS*PPS*RSPR - 0.70 0.77
Aak1 S635 AP2-associated protein kinase 1 ILS*DVTHSAVFGVPASK GHRRILS*DVTHSA 0.62 0.48
Map3k2 S331, S344 Mitogen-activated protein kinase kinase kinase 2 RRGS*DIDNPTLTVTDIS*PPSRSPR - 0.62 0.48
Map3k2 S331, S349 Mitogen-activated protein kinase kinase kinase 2 RGS*DIDNPTLTVTDISPPSRS*PR - 0.54 1.01
Prkd2 S211, S214 Serine/threonine-protein kinase D2 LGS*SES*LPCTAEELSR - 0.47 1.27
Srpk1 S450 SRSF protein kinase 1 ADTPS*GDEQEPNGALDSK IRADTPS*GDEQEP 0.44 0.66
Prkd2 S197, S206 Serine/threonine-protein kinase D2 RLS*STSLASGHS*VR - 0.42 0.66
Prkd2 S197, S198 Serine/threonine-protein kinase D2 RLS*S*TSLASGHSVR - 0.42 1.11
Nek4 S430 Serine/threonine-protein kinase Nek4 RSS*DGGDGEGSELVKPLYPSNK LLPRRSS*DGGDGE 0.41 0.59
Erbb2 S1055 Receptor tyrosine-protein kinase erbB-2 S*GGGELTLGLEPSEEEPPR RSSSARS*GGGELT 0.40 0.61
Src S74 Neuronal proto-oncogene tyrosine-protein kinase Src LFGGFNSSDTVTS*PQR SSDTVTS*PQRAGP −0.40 0.40
Map3k3 S147 Mitogen-activated protein kinase kinase kinase 3 IKPSQS*AGDINTIYQAPEPR RIKPSQS*AGDINT −0.42 0.60
Mapk3 Y205 Mitogen-activated protein kinase 3 IADPEHDHTGFLTEY*VATR TGFLTEY*VATRWY −0.44 1.44
Taok1 S421 Serine/threonine-protein kinase TAO1 ASDPQS*PPQVSR RASDPQS*PPQVSR −0.44 0.54
Map3k5 S965 Mitogen-activated protein kinase kinase kinase 5 LSALSTGS*NEYLR SALSTGS*NEYLRS −0.48 0.47
Map3k11 S508 Mitogen-activated protein kinase kinase kinase 11 ITVQAS*PGLDR RITVQAS*PGLDRR −0.54 0.66
Csnk1d T349 Casein kinase I isoform delta GTQEVAPPTPLTPT*SHTANTSPRPVSGMER PTPLTPT*SHTANT −0.60 0.48
Mark3 S583 MAP/microtubule affinity-regulating kinase 3 TATYNGPPAS*PSLSHEATPLSQTR YNGPPAS*PSLSHE −0.70 0.98
Mylk S355 Myosin light chain kinase, smooth muscle VPAIGSFS*PGEDRK PAIGSFS*PGEDRK −0.73 0.59
Mylk S935 Myosin light chain kinase, smooth muscle KVHS*PQQVDFR EERKVHS*PQQVDF −0.76 0.62
Mylk S355 Myosin light chain kinase, smooth muscle VPAIGSFS*PGEDR PAIGSFS*PGEDRK −0.79 0.56
Mink1 S746 Misshapen-like kinase 1 LDSS*PVLSPGNK STKLDSS*PVLSPG −0.84 0.42
Peak1 T1141 Inactive tyrosine-protein kinase PEAK1 TDQEGLNASQPT*PPPLPK LNASQPT*PPPLPK −0.88 0.41
Lmtk2 S1418 Serine/threonine-protein kinase LMTK2 YFS*PPPPAR QTSKYFS*PPPPAR −0.93 0.53
Map3k2 S344, S347 Mitogen-activated protein kinase kinase kinase 2 GSDIDNPTLTVTDIS*PPS*RSPR - −1.45 0.45
Map3k2 S347 Mitogen-activated protein kinase kinase kinase 2 GSDIDNPTLTVTDISPPS*RSPR TDISPPS*RSPRAP −1.57 0.74

The complete list of 9640 phosphopeptides was filtered based on dual criteria (−Log10(CV)≥0.4 and |Log2(dDAVP/Vehicle) |>0.4) to shortlist 25 protein kinases (36 phosphopeptides) with high probability abundance changes in response to dDAVP. This table contains mono, and multi-phosphorylated peptides. For multi-phosphorylated peptides, no centralized sequence is presented. The mean ratio from three clones of mpkCCD cells used for three SILAC experiments were presented. CV = Coefficient of variation in vasopressin response among three clones of mpkCCD cells.