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Zymogram of purified POXA1b incubated with 7-day filtered culture broth (100 μl). Lane 1, control collected at zero time; lane 2, sample collected after 2 days of incubation without protease inhibitors; lane 3, sample collected after 2 days of incubation in the presence of protease inhibitors. One-microliter aliquots were loaded into the lanes.
