Fig. 5.
Recombinant DYRK2 activates αS617AβγENaC. a and b, left panels, representative current traces recorded in outside-out patches of αS617AβγENaC expressing oocytes as described in Fig. 2. Heat-inactivated DYRK2 (inactive DYRK2) or active recombinant DYRK2 (80 U/ml) were included in the pipette solutions as indicated under the traces. Right panels, summary of normalized ∆IAmi values obtained from similar experiments as shown in the representative traces (left panels) using the same symbols as in Fig. 2. αS617AβγENaC/inactive DYRK2, n = 3; αS617AβγENaC/DYRK2, n = 6. ∆IAmi values determined at individual time points were compared with the corresponding initial ∆IAmi value at 4 min using paired Student’s ratio t-test. ** p < 0.01