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. 2000 Mar;66(3):925–929. doi: 10.1128/aem.66.3.925-929.2000

FIG. 1.

FIG. 1

Electrophoresis of the purified laccase from M. quercophilus. (A) SDS–7.5% PAGE of purified laccase from M. quercophilus. Lane 1, standard proteins; lane 2, 20 μg of crude extract; lane 3, 10 μg of purified laccase. (B) Isoelectric focusing of purified laccase from M. quercophilus. Lane 1, laccase; lane 2, standards. Proteins were stained with Coomassie brilliant blue. (C) Native PAGE of purified laccase from M. quercophilus. Lane 1, 1 μg of purified laccase; lane 2, 3 μg of the same sample. Protein was stained with guaiacol. (D) Lanes 1 and 3, standard proteins; lane 2, untreated laccase; lane 4, laccase treated with N-glycanase. Proteins were stained with Coomassie brilliant blue. Dried gels were scanned with an Agfa Snapscan 1236 piloted with Fotolook 2.09.6 software. Legends were added with Canvas 3.0.6 software.