TABLE 2.
Comparison of phenolic compound oxidation in the crude extract versus laccase 1 purified solution
| Substrate | O2/substrate (mol/mol)a
|
|
|---|---|---|
| Laccase 1 | Crude extract | |
| ABTS | 0.24 ± 0.01 | 0.26 ± 0.01 |
| Catechol | 0.56 ± 0.02 | 0.53 ± 0.02 |
| 4-Methyl catechol | 0.40 ± 0.01 | 0.55 ± 0.01 |
| Protocatechuic acid | 0.40 ± 0.00 | 0.59 ± 0.02 |
| Guaiacol | 0.47 ± 0.02 | 0.58 ± 0.02 |
| Coniferylic acid | 0.66 ± 0.02 | 0.76 ± 0.03 |
| Coumarylic alcohol | 0.48 ± 0.02 | 0.37 ± 0.02 |
| Sinapylic alcohol | 0.51 ± 0.04 | 0.91 ± 0.04 |
| Ferulic acid | 0.40 ± 0.04 | 0.64 ± 0.03 |
| Gallic acid | 1.00 ± 0.03 | 1.28 ± 0.05 |
| Caffeic acid | 0.40 ± 0.02 | 0.59 ± 0.03 |
| Veratrylic alcohol | 0 | 0 |
a
Values are means from three experiments.