TABLE 1.
Characterization of primers used for SSCP-based microbial-community analyses
| Primer name | Primer sequence (5′–3′) | Primer target (positions)a | Targeted variable region(s) of the small-subunit rRNA genesb | Primer annealing temp during PCR (°C) | Reference |
|---|---|---|---|---|---|
| Com1 | CAG CAG CCG CGG TAA TAC | Bacteria (519–536) | V4 and V5 | 50 | 41 |
| Com2 | CCG TCA ATT CCT TTG AGT TT | Bacteria (907–926) | |||
| F243 | GGA TGA GCC CGC GGC CTA | Actinomycetes (226–243) | V3 | 60 | 22 |
| R531 | CGG CCG CGG CTG CTG GCA CGT A | Actinomycetes (512–534) | |||
| NS7 | GAG GCA ATA ACA GGT CTG TGA TGC | Fungi (1185–1207) | V8 and V9 | 50 | 49 |
| NS8 | TCC GCA GGT TCA CCT ACG GA | Fungi (1508–1527) |