TABLE 2.
Characterization of molecular isolates, cloned from PCR-SSCP genetic profiles of 16S rRNA genes amplified from total DNA extracted from compost
| Primer-targeted gene sequence (region) | Clone | GenBank accession no. | Closest relative based on partial sequence homologya (% similarity) | Age of composting sample used for product isolation (days) | Period of product detection in SSCP community profiles (days) |
|---|---|---|---|---|---|
| 16S rRNA (V4 and V5) | CB-2 | AF213263 | Lactobacillus panis (94.9) | 4 | 2–4 |
| CB-1 | AF213262 | Lactobacillus confusus (99.4) | 2 | 2–6 | |
| CB-3 | AF213264 | Candida krusei (99.6) | 6 | 2–10 | |
| CB-9 | AF213270 | Bacillus thermocatenulatus (92.3) | 10 | 2–18 | |
| CB-12 | AF213273 | Bacillus badius (100.0) | 2 | 2–18 | |
| CB-6 | AF213267 | Azotobacter salinestris (97.1) | 6 | 4–12 | |
| CB-5 | AF213266 | Xanthomonas campestris (96.8) | 6 | 4–12 | |
| CB-7 | AF213268 | Bacillus stearothermophilus (98.1) | 10 | 6–10 | |
| CB-4 | AF213265 | Bacillus caldoxylolyticus (98.6) | 6 | 6–12 | |
| CB-10 | AF213271 | Clostridium thermolacticum (94.1) | 18 | 6–18 | |
| CB-8 | AF213269 | Bacillus fusiformis (96.1) | 10 | 10 | |
| CB-11 | AF213272 | Microbispora bispora (99.2) | 15 | 15–18 | |
| 16S rRNA (V3) | CA-4 | AF213277 | Streptomyces nodosus (98.3) | 10 | 2–15 |
| CA-5 | AF213278 | Streptomyces thermodiastaticus (100.0) | 12 | 2–15 | |
| CA-3 | AF213276 | Streptosporangium vulgare (98.2) | 15 | 2–15 | |
| CA-1 | AF213274 | Bacillus thermoleovorans (92.4) | 12 | 4–12 | |
| CA-2 | AF213275 | Detolaasinbacter sp. (99.0) | 15 | 4–15 | |
| 18S rRNA (V8 and V9) | CP-1 | AF213279 | Candida krusei (99.4) | 4 | 1–18 |
| CP-2 | AF213280 | Candida tropicalis (98.4) | 15 | 10–18 |
a
Identified by BLASTN 2.0.8 (1).