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. 2022 Jun 13;13(6):547. doi: 10.1038/s41419-022-04983-7

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A Heatmap showed occupancy for KDM2A across the genome in UMUC3 cells. B Genome-wide distribution of KDM2A ChIP-seq peaks in UMUC3 cells. C Cumulative distribution curve shows KDM2A activating or repressing effects. Differential genes obtained from RNA-seq after KDM2A KD were divided into three groups: upregulated, downregulated, and unchanged according to the expression pattern. The corresponding genes of KDM2A ChIP-seq were sorted from high to low according to regulatory potential score, and the cumulative distribution curves of upregulated, downregulated, and invariant genes were made. D GO terms of upregulated genes that KDM2A target directly. E Heatmap showed the ranking of upregulated cytokine activity genes upon KDM2A KD. F Genome browser tracks of KDM2A ChIP-seq data and RNA-seq data in RARRES3 gene loci. G The protein level of KDM2A, RARRES3, and EpCAM (the biomarker of bladder cancer) by immunofluorescence staining assays. H ChIP-qPCR showed the H3K36me2 modification of RARRES3 promoter upon KDM2A KD. *P < 0.05; **P < 0.01; ***P < 0.001 is based on the Student’s t test. All results are from more than three independent experiments. Values are mean ± SD.