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. 2022 Jun 13;13:3385. doi: 10.1038/s41467-022-31009-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Representative fluorescence images of a captured LNCaP cluster spiked into unprocessed whole blood. Following the PBS wash and fixation, the isolated tumor cells were stained with Cytokeratin (green) and DAPI (nuclei, blue). Scale bars, 20 μm. b Schematic illustration of the experimental setup used for the characterization studies. The cells entering and exiting the analytical versions of the Cluster-Wells were visually tracked and counted for capture efficiency calculations using a 2-channel microfluidic interface (see “Methods”: measurement of the device sensitivity). c Cluster capture efficiencies for the spiked LNCaP clusters at different flow rates. Data is provided according to the number of cells in clusters (n = 3 independent experiments). Data are presented as mean ± SD. d Two-cell cluster capture rates for prostate (LNCaP), breast (MDA-MB-231 & MCF-7), and ovarian (HeyA8) cancer cell clusters at the flow rate of 25 mL/h (n = 3 independent experiments). Data are presented as mean ± SD. Since the Cluster-Wells solely relies on physical attributes of clusters, it efficiently captured clusters of different tumor types, independent of their surface antigens. e Two, three, and four-cell LNCaP cluster capture efficiencies for the devices with different opening sizes (n = 3 independent experiments). Experiments were performed at 25 mL/h flow rate. Data are presented as mean ± SD. f White blood cell (WBC) retention rates for the devices with different opening sizes after processing a tube (10 mL) of unprocessed whole blood (n = 3 independent experiments). The non-specifically attached WBCs were stained and counted. For the calculation of retention rate, obtained WBC count was divided by the total number of WBCs ran through the device, which was acquired from a complete blood count (CBC) analyzer. Data are presented as mean ± SD. Source data are provided as a Source Data file.