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. 2022 Jun 13;13:3385. doi: 10.1038/s41467-022-31009-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Micromanipulation of a CTC cluster isolated from the blood sample of a metastatic prostate cancer patient (Patient-1). Following the blood filtration and PBS wash, isolated viable CTC clusters were stained on the device using FITC-conjugated antibodies against EpCAM and prostate specific membrane antigen (PSMA), scanned using fluorescence microscope, and micromanipulated directly from the device. Scale bars, 50 μm. b Representative fluorescence microscope images of the prostate CTC clusters that are subjected to RNA-Seq. Images were taken before they were retrieved from the device for molecular analysis. Scale bars, 20 μm. c t-distributed stochastic neighbor embedding (t-SNE) plot of the sequenced patient clusters, prostate cancer cell lines, and white blood cells. d Heatmap plot illustrating the expression levels of selected genes in isolated CTC clusters from two metastatic prostate cancer patients, prostate cancer cell lines, and white blood cells. RPM reads per million.