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. 2022 Jun 13;13(6):548. doi: 10.1038/s41419-022-04997-1

Fig. 5. Promotion of the Hsp90-Acsl4 pathway enhances Acsl4-dependent ferroptosis.

Fig. 5

A, B Expression levels of proteins in Hsp90-Acsl4 pathway were determined by western blot in the indicated groups. Data indicated as mean ± S.D. (n = 5 experiments). C Confocal images showed colocalization of oxidized lipids (green) and mitochondria (red). PL1 cells of indicated groups were treated with erastin (1 μM, 6 h), and PG7 cells of the indicated groups were treated with erastin (2 μM, 6 h); then the cells were stained with BODIPY C11 and MitoTracker. Scale bar: 10 μm. D–I Intracellular ROS, MDA, 12-HETE,15-HETE levels, and GSH and GPX activity in PL1 and PG7 cells treated as indicated before. Data indicated as mean ± S.D. (n = 5 experiments). *p < 0.05, **p < 0.01, ***p < 0.001.