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. 2022 Jun 13;12:9723. doi: 10.1038/s41598-022-13839-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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The rGA733-Fc protein purification and glycosylation pattern analysis. rGA733-Fc protein was purified from the transgenic tomato plants using a protein A column. (a) The eluted fractions were separated by SDS-PAGE and visualized by Coomassie brilliant blue staining. M, protein size marker; T, total soluble protein; E, purified rGA733-Fc protein. (b) rGA733-Fc protein in 5th eluted fraction was detected with the rGA733-2 antibody by western blot analysis. 5th E, 5th eluted fraction. (c) Glycosylation patterns of purified rGA733-Fc protein were analyzed by Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). Blue square, N-acetylglucosamine (GlcNAc); green circle, mannose.