Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 13;7:185. doi: 10.1038/s41392-022-00985-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — The regulation of NIBP by RNF138 is independent of the Ub-E3 ligase activity. a Schematic representation of RNF138 mutant constructs. Asterisk indicates amino acid residue mutant location (C/A) in RING domain. b Schematic representation of NIBP truncation constructs map. c Co-IP analysis of the interaction between HA-NIBP and full-length or truncated Flag-RNF138 mutants in 293T cells. d Co-IP analysis of the interaction between RNF138 and Flag-NIBP truncations in 293T cells. e Representative images of NIBP-immunostaining in HCT116 and RKO RNF138^KO cell lines transfected with empty vector or RNF138 truncations (flag-red), with co-localization as shown by the pixel intensity profile. Quantification of NIBP nucleus intensity in HCT116 and RKO cells transfected with different RNF138 mutants (right). Scale bar, 5 μm. f Immunoblots of phosphorylation and total p65 levels in HCT116 and RKO-knockout cells transfected with EV or different RNF138 truncations. g qPCR analysis of NF-κB target gene expression in HCT116 and RKO-knockout cells transfected with EV or RNF138 truncations. h Cell viability of HCT116- and RKO-knockout cells transfected with EV or RNF138 truncations