Figure 3.

Preparation and characterization of AuNPs-PEI-pDNA

(A) Gel retardation assay after AuNPs-PEI combining with pBI121-GFP, pCB302-NPR1-GFP, and pBI221-NLS-mCherry, respectively. As the concentration of AuNPs-PEI increased, the DNA band gradually weakened.

(B) Changes in zeta potential before and after AuNPs-PEI and pNPR1-GFP binding. Owing to the cancellation of the positive and negative potentials, the zeta potential of the AuNPs-PEI surface dropped by about 12mV after binding. Data were represented as means ± SD from three repeats.

(C) TEM images of AuNPs-PEI-pNPR1-GFP. The particle size of AuNPs-PEI did not change significantly after binding the plasmid.