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. 2022 May 29;25(6):104479. doi: 10.1016/j.isci.2022.104479

Figure 5.

Figure 5

The process of AuNPs-PEI-FAM-siRNANPR1 entry into the nucleus

(A) Gel retardation assay after AuNPs-PEI combining with FAM-siRNANPR1. As the concentration of AuNPs-PEI increased, the RNA band gradually weakened.

(B) Co-localization of FAM-siRNANPR1 fluorescence and NLS-mCherry fluorescence in healthy periwinkle leaves 2 days after AuNPs-PEI-FAM-siRNANPR1 infiltration. The green fluorescence was the fluorescence of FAM-siRNANPR1. The red fluorescence was the autofluorescence of chlorophyll. The yellow fluorescence was the fluorescence of NLS-mCherry. Scale bars, 10 μm.

(C) Confocal observation of healthy periwinkle leaves after AuNPs-PEI-FAM-siRNANPR1 infiltration at different time points. AuNPs-PEI-pNLS-mCherry was infiltrated 2 h in advance. The green fluorescence was the fluorescence of FAM-siRNANPR1. The red fluorescence was the autofluorescence of chlorophyll. The yellow fluorescence was the fluorescence of NLS-mCherry. Scale bars, 10 μm.