Antibodies |
|
anti-GFP (JL-8 Monoclonal Antibody) |
Invitrogen |
CAT#A-6455; RRID: AB_221570
|
anti-ACTIN |
Sigma |
CAT#A0480; RRID:AB_476670
|
Goat anti-mouse IgG-HRP |
Absin |
CAT#abs20001; RRID: AB_2716555
|
|
Chemicals, peptides, and recombinant proteins |
|
Polyethylenimine (PEI) |
Shyuanye |
CAT#S24467-25mL |
Gold chloride trihydrate |
Aladdin |
CAT#G141105-1g |
|
Critical commercial assays |
|
Trizol |
Takara |
CAT#9109 |
Hifair® Ⅱ 1st Strand cDNA Synthesis Kit |
Yeasen Biotechnology |
CAT#11139ES60 |
Hieff® qPCR SYBR Green Master Mix (Low Rox) |
Yeasen Biotechnology |
CAT#11202ES08 |
EZ-10 Spin Column Plant Genomic DNA Purification Kit |
Sangon |
CAT#B518261-0050 |
2X TaqMan Fast qPCR Master Mix |
Sangon |
CAT#B639274-0005 |
|
Experimental models: Organisms/strains |
|
Arabidopsis thaliana ecotype Col-0 |
N/A |
N/A |
Catharanthus roseus |
N/A |
N/A |
|
Recombinant DNA |
|
pBI221-NLS-mCherry |
Li et al. (2019a) |
N/A |
pBI121-GFP |
Li et al. (2019a) |
N/A |
pCB302-AtNPR1-GFP |
Chen et al. (2019) |
N/A |
|
Software and algorithms |
|
Graphpad Prism |
Graphpad |
N/A |
Excel |
Microsoft |
N/A |
ZEN |
Carl Zeiss |
N/A |